Nucleic acids and proteins have proven to be extremely useful biomarkers for disease diagnosis, especially early disease detection. PCR-based nucleic acid amplification tests were the gold-standard for confirmed COVID-19 diagnosis, which became a household term during the pandemic.
This course is designed to equip learners with the essential skills required to develop biosensors which enable disease identification by detecting specific nucleic acid sequences and/or proteins. Significant focus will be on discussions for developing point-of-care diagnostics. A non-exhaustive list of techniques discussed in this course includes PCR, isothermal nucleic acid amplification assays, nucleic acid/protein-based lateral flow assays, oligo ligation assays, ELISA, sandwich ELISA, probe-based assays, dipstick assays, and CRISPR-based assays.
This course is designed to equip learners with the essential skills required to develop biosensors which enable disease identification by detecting specific nucleic acid sequences and/or proteins. Significant focus will be on discussions for developing point-of-care diagnostics. A non-exhaustive list of techniques discussed in this course includes PCR, isothermal nucleic acid amplification assays, nucleic acid/protein-based lateral flow assays, oligo ligation assays, ELISA, sandwich ELISA, probe-based assays, dipstick assays, and CRISPR-based assays.
Project plan:
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The class engrossed in a graded quiz while enjoying the sun and breeze on a pleasant winter morning.