Call for master’s thesis project applications
Title: Developing a multiplexed CRISPR assay to enable field detection of fungal diseases in crops
Objective 1: Culturing of fungal strains, DNA isolation, and quantification
Techniques:
Bioinformatics, Gel electrophoresis, Nanodrop, Spectrophotometry, Genomic DNA isolation, PCR, fungal cell culture etc.
Skills Required:
Objective 1: Culturing of fungal strains, DNA isolation, and quantification
- Optimizing nutrient media and growth conditions for culturing selected fungal strains
- Genomic DNA isolation and quality testing
- Creating a standardized protocol to ensure consistency of the experimental set-up
- Bioinformatics study to distinctively identify selected fungal strains
- Designing target-specific guide RNAs (gRNAs) and selecting appropriate Cas enzymes
Techniques:
Bioinformatics, Gel electrophoresis, Nanodrop, Spectrophotometry, Genomic DNA isolation, PCR, fungal cell culture etc.
Skills Required:
- Molecular and cell biology lab techniques like media preparation, plating, streaking, spreading, sample preparation, DNA extraction, PCR, and gel electrophoresis.
- Knowledge of basic databases and bioinformatics tools like NCBI, Uniprot, PubMed, and BLAST
- Strong interpersonal skills to collaborate with team members
- Strong oral and written communication skills to share findings, present results and prepare detailed documentation
- Ability to design and troubleshoot experiments, and interpret experimental data
- Experience of working with fungal strains will be beneficial